RAW264.7 대식세포에서 파파야 및 오크라 추출물의 면역활성 증진 효과
- Alternative Title
- Immunomodulating activity of Carica papaya and Abelmoschus esculentus extracts in mouse RAW 264.7 macrophages
- Abstract
- The recent global spread of COVID-19 has resulted in high levels of morbidity and mortality. Since then, as it has been found that lowering immunity affects the spread of COVID-19, the importance of immunity is getting more attention. Therefore, many researchers are developing natural products that have an excellent effect on regulating the immune function of the body or strengthening the immune system. In this study, papaya leaf water extract (PLW) and okra leaf water extract (OLW) were found to significantly increase the levels of nitric oxide (NO) and prostaglandin E2 (PGE2) by upregulating inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) activity, respectively. Additionally, PLW and OLW increased the production of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in RAW 264.7 cells. Furthermore, PLW and OLW activated the expression of c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase and extracellular signal-regulated kinase (ERK). These results indicate that PLW and OLW increased the production of NO, PGE2, and pro-inflammatory cytokines by activating the JNK, p38 and ERK pathways in RAW 264.7 macrophages, thus demonstrating immunomodulatory properties. Finally, several metabolites such as flavonoid, alkaloid present in PLW and OLW also were analyzed through UPLC-Q-TOF MS in this study. Taken together, this study shows that PLW and OLW can act as immunoactive factors that stimulate and activate RAW 264.7 cells. In addition, it was finally confirmed that the MAPK and NF-κB signaling pathways are involved in this immune activity enhancing effect. Therefore, it is judged that PLW and OLW can be developed as natural materials with immune activation effects.
- Author(s)
- 고민녕
- Issued Date
- 2022
- Awarded Date
- 2022. 2
- Type
- Dissertation
- URI
- https://dcoll.jejunu.ac.kr/common/orgView/000000010476
- Alternative Author(s)
- Ko, Min Nyeong
- Affiliation
- 제주대학교 대학원
- Department
- 대학원 화학과
- Advisor
- 현창구
- Table Of Contents
- ABSTRACT 1
목 차 2
LIST OF TABLES 4
LIST OF FIGURES 5
Ⅰ. 서 론 7
Ⅱ. 재료 및 방법 11
1. 시료 및 시약 11
2. 실험방법 12
2.1. 추출물 제조 12
2.2. 세포 배양 13
2.3. 세포 생존율 측정 14
2.4. NO 생성량 측정 15
2.5. Prostaglandin E2 (PGE2) 및 전염증성 cytokine (TNF-α, IL-1β) 생성량 측정 16
2.6. Western blot 17
2.7. UPLC-Q-TOF MS 분석 18
3. 통계처리 20
Ⅲ. 결 과 21
1. 세포 생존율 측정 21
2. NO 생성량 측정 23
3. Prostaglandin E2 (PGE2) 생성량 측정 25
4. 전염증성 cytokine (TNF-α, IL-1β) 생성량 측정 27
4.1. TNF-α 생성량 측정 28
4.2. IL-1β 생성량 측정 30
5. Western blot 32
5.1. PLW와 OLW의 iNOS 발현에 미치는 효과 32
5.2. PLW와 OLW의 COX-2 발현에 미치는 효과 34
5.3. PLW와 OLW의 MAPK 발현에 미치는 효과 36
5.4. PLW와 OLW의 NF-κB 발현에 미치는 효과 39
6. 대사산물 분석 43
Ⅳ. 고 찰 48
Ⅴ. 참고문헌 53
- Degree
- Master
- Publisher
- 제주대학교 대학원
-
Appears in Collections:
- General Graduate School > Chemistry
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.