A synthetic Isoflavone, DCMF, Promotes Human Keratinocyte Migration by Activating Src/FAK Signaling Pathway
- Alternative Title
- 새로운 플라보노이드 물질 (DCMF)의 Src/FAK 경로 활성화를 통한 각질세포의 이동성 증가 효과
- Abstract
- Flavonoids are a class of secondary compounds produced by plants that contain various pharmacological properties. Our previous report showed that TMF, trimethoxyisoflavone, provided a good effect on wound healing by inducing keratinocyte migration. In this study, we screened derivatives of TMF for searching better pharmacological effects and found 2',6 Dichloro-7-methoxyisoflavone (DCMF) is better candidate for wound healing agents. We investigate the effects and possible action mechanism of 2',6 Dichloro-7-methoxyisoflavone (DCMF) on wound healing using keratinocyte HaCaT cell line and in excisional wound animal model. We found DCMF markedly increased keratinocyte cell migrations but not proliferation. DCMF induced activation of ERK, AKT and p38 MAPK signaling pathways through Src kinase. We also found DCMF induced secretion of MMP-2 and MMP-9, and partial epithelial-mesenchymal transition (EMT). Finally, effects induced by DCMF were abolished when Src was inhibited. In in vivo excisional wound model, DCMF treated mice showed improvement of wound closure and re-epithelialization. In conclusion, DCMF induces cell migration through activation Src, ERK, AKT, p38 signaling pathway.
- Author(s)
- Phorl Sophors
- Issued Date
- 2015
- Awarded Date
- 2016. 2
- Type
- Dissertation
- URI
- http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007536
- Alternative Author(s)
- 폴 소폴스
- Department
- 대학원 의학과
- Advisor
- 조문제
- Table Of Contents
- 1. Abstract . 1
2. Introduction . 2
3. Material and Method . 4
3.1. Synthesized DCMF and Antibodies 4
3.2. Culture of HaCaT cells 4
3.3. Scratch Wound Healing Assay 4
3.4. MTT 5
3.5. Cell Counting 5
3.6. Western Blot . 5
3.7. Wounding Experiment 6
3.8. Histological Analyses 6
3.9. Statistical analysis. 6
4. Result 7
4.1. DCMF promotes HaCaT cell migration via activation of Src. . 7
4.2. DCMF activates Src to mediate phosphorylation of ERK, AKT and p38 MAPK 16
4.3. DCMF promotes MMP-2 and MMP-9 Secretion . 21
4.4. DCMF induces change EMT regulatory molecules via Src 23
4.5. DCMF improves wound healing and re-epithelialization . 25
5. Discussion . 27
6. References . 31
- Degree
- Master
- Publisher
- 제주대학교 대학원
- Citation
- Phorl Sophors. (2015). A synthetic Isoflavone, DCMF, Promotes Human Keratinocyte Migration by Activating Src/FAK Signaling Pathway
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- General Graduate School > Medicine
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